Non-Invasive Visualization of Epidermal Responses to Injury Using a Fluorescent Transgenic Reporter

Rapid non-invasive genotyping of reporter transgenic mammals.

Here we describe a non-invasive method for rapid and highly reproducible genotyping of transgenic mammals with ubiquitous expression of fluorophore reporters. Hair samples from transgenic mice and pigs with systemic expression of the fluorophore reporter Venus were analyzed with a fluorescence microscope in few minutes. The hair samples can be preserved for long-term storage at ambient temperat...

Non-invasive imaging using reporter genes altering cellular water permeability

Non-invasive imaging of gene expression in live, optically opaque animals is important for multiple applications, including monitoring of genetic circuits and tracking of cell-based therapeutics. Magnetic resonance imaging (MRI) could enable such monitoring with high spatiotemporal resolution. However, existing MRI reporter genes based on metalloproteins or chemical exchange probes are limited ...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Flow Visualization Around a Non - Circular Tube

The flow behavior around a cam shaped tube in cross flow has been investigated experimentally using flow visualization and pressure distribution measurement. The range of attack angle and Reynolds number based on an equivalent circular diameter are within 0 ≤ α ≤ 360˚ and 2 x 104 < Reeq < 3.4 x 104, respectively. The pressure drag features are clarified in relation to the flow behavior around t...

Non-invasive visualization of the lipid product of class I PI3K in transgenic mouse models.

PI3Ks (phosphoinositide 3-kinases) regulate many critical cellular responses by producing PI(3,4,5)P(3) (phosphatidylinositol 3,4,5-trisphosphate). To facilitate the spatio-temporal characterization of PI(3,4,5)P(3) in living primary cells, we generated a novel strain of transgenic mice [AktPH (Akt pleckstrin homology domain)-GFP (green fluorescent protein) Tg (transgenic) mice] that express a ...